Lukeman Lab
Presented by Team Toehold Conga Nanny
Our Positioner Journey
In the assembly of our positioner, p7560 is used as a single-strand scaffold DNA. This scaffold is folded into the positoner structure (Figure 1) with the help of a variety of staple strands. The length of the adjuster can be varied by changing the number of bases, so the angle between the arm and the the base will also change.
Figure 1. Our modified positioner; side view. Black Bars refer to pairs of helices, antiparallel arrows refer to the adjustable duplexes.
Cadnano
Cadnano is a tool for designing structures of DNA origami. It was used as a method to systematically represent the origami that we are producing, and to tidily create variations of the origami we were working with. This was used to show and archive the different formations of both the triangle and the claw. These Cadnano representations are useful in record keeping, and provide a simple way to produce strands for purchase.
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The cadnano representation of our positioner is shown in Figure 2.
Figure 2. Cadnano design representation of the Positioner
Positioner Strand's Sequences
USING DNA ORIGAMI FOR
A NANOSCALE ELECTROCHEMICAL POSITIONER MODEL
Presented by Team Toehold Conga Nanny